FIG. 5.

Aconitase B is highly sensitive to oxidants in cell extracts, but AcnA is resistant. (A) Concentrated SOD-deficient extracts containing AcnB (SMV3) (squares) or AcnA (SMV7) (circles) were exposed to oxygen (open symbols) and superoxide (closed symbols) as described in Materials and Methods. (B) Concentrated catalase-deficient extracts containing AcnB (SMV11) (squares) or AcnA (SMV11) (circles) were exposed to 4 mM H₂O₂ anaerobically. (C) Concentrated extracts containing AcnB (JRG2789) (squares) and AcnA (JRG3258) (circles) were exposed to 400 μM ferricyanide. Time courses are representative of at least three experiments.