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Purification of STM4066
| Purification step | Protein amt (mg) | Activity (nmol/ min) | Sp act (nmol/ min/mg) | % Yield | Fold purifi- cation |
|---|---|---|---|---|---|
| Crude extract | 240 | 5,832 | 24.3 | 100 | 1 |
| Clarified crude extract | 258 | 5,934 | 23.0 | 102 | 0.95 |
| 30-50% (NH4)2SO4 fraction | 108 | 2,862 | 26.5 | 49 | 1.1 |
| DEAE-Sepharose Fast Flow | 10.8 | 844.6 | 78.2 | 14 | 3.2 |
| Superdex 200 preparative grade | 3 | 413.1 | 137.7 | 7 | 5.7 |
