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Phosphorylation of the OmpRN-PhoBC chimeras by EnvZ. The kinase assays are described in Materials and Methods. The phosphotransfer reaction was performed for 1 h at room temperature and stopped by the addition of SDS-PAGE loading buffer. Lane 1 contains 2 μg of EnvZ autophosphorylated by [γ-32P]ATP. The response regulator (RR; 0.3 μg) in each reaction is indicated by the labels under the gel.
