TABLE 2.

Relative fold changes of transcript amounts of genes that encode ribosomal proteins, AARSs, and amino acid biosynthetic enzymes in response to sublethal concentrations of translation inhibitors

Functional category and gene	Protein	Entry no. in R6 genome database	Avg relative fold changea
			Ermb	Cmb	Purb	Tetb
Ribosomal proteins
rpsD	30S ribosomal protein S4	spr0078	1.9	1.1	1.1	2.8
rpsJ	30S ribosomal protein S10	spr0187	1.4	1.6	2.1	1.8
rplC	50S ribosomal protein L3	spr0188	1.5	1.5	2.0	1.8
rplD	50S ribosomal protein L4	spr0189	1.6	1.8	2.0	2.0
rplW	50S ribosomal protein L23	spr0190	1.6	1.7	2.1	2.0
rplB	50S ribosomal protein L2	spr0191	1.6	1.7	2.0	1.9
rpsS	30S ribosomal protein S19	spr0192	1.7	1.8	2.1	2.1
rplV	50S ribosomal protein L22	spr0194	1.8	2.4	2.1	1.9
rplP	50S ribosomal protein L16	spr0196	1.7	2.0	2.0	1.8
rpmC	50S ribosomal protein L29	spr0197	1.7	2.0	2.0	1.7
rplX	50S ribosomal protein L24	spr0200	1.9	2.4	2.0	2.2
rplE	50S ribosomal protein subunit L5	spr0201	1.7	2.1	2.0	2.0
rplF	50S ribosomal protein subunit L6	spr0204	1.7	2.0	2.0	2.1
rplR	50S ribosomal protein L18	spr0205	1.6	1.9	2.0	1.9
rplK	50S ribosomal protein L11	spr0555	1.3	2.2	1.8	1.6
rpsP	30S ribosomal protein S16	spr0682	1.4	1.7	−1.1	2.1
rpmI	50S ribosomal protein L35	spr0862	1.3	2.3	1.7	2.3
rplT	50S ribosomal protein L20	spr0863	1.6	2.9	1.9	3.2
rplJ	50S ribosomal protein L10	spr1212	1.0	1.7	1.6	1.9
rpsR	30S ribosomal protein S18	spr1394	2.3	2.4	1.9	2.9
AARSs
serS	Seryl-tRNA synthetase	spr0372	−1.9	−2.2	−1.8	−2.0
valS	Valyl-tRNA synthetase	spr0492	−3.0	−2.1	−1.5	−2.5
pheS	Phenylalanyl-tRNA synthetase alpha chain	spr0507	−13.2	−4.7	−2.3	−13.5
pheT	Phenylalanyl-tRNA synthetase beta chain	spr0509	−2.7	−2.4	−2.1	−4.0
glyS	Glycyl-tRNA synthetase beta chain	spr1328	−2.2	−1.9	−1.4	−2.3
glyQ	Glycyl-tRNA synthetase alpha chain	spr1329	−2.5	−2.8	−1.8	−2.7
thrS	Threonyl-tRNA synthetase 1	spr1472	−2.3	−1.9	−1.2	−2.1
ileS	Isoleucyl-tRNA synthetase	spr1502	−3.6	−2.4	−1.5	−3.3
tyrS	Tyrosyl-tRNA synthetase 1	spr1910	−2.2	−2.0	−1.4	−1.5
hisS	Histidyl-tRNA synthetase	spr1931	−2.2	−1.5	−1.4	−1.7
Translation factors
prfC	Peptide chain release factor 3	spr0396	1.2	2.0	1.2	−1.1
infC	Translation initiation factor 3	spr0861	1.4	2.1	1.8	2.4
Amino acid biosynthesis
aspC	Aspartate aminotransferase	spr0035	−1.8	−2.1	−1.4	−1.4
ilvN	Acetolactate synthase small subunit	spr0402	−3.2	−3.3	−3.2	−5.1
ilvC	Ketol acid reductoisomerase	spr0403	−2.2	−2.9	−3.4	−3.2
metE	Tetrahydropteroyltriglutamate methyltransferase	spr0514	−6.5	−8.1	−4.2	−5.4
metF	5,10-Methylenetetrahydrofolate reductase	spr0515	−4.5	−3.8	−3.5	−3.6
ilvE	Branched-chain-amino-acid transaminase	spr0758	−3.1	−2.5	−2.3	−3.9
asd	Aspartate beta-semialdehyde dehydrogenase	spr0918	−3.0	−3.1	−2.5	−3.8
dapA	Dihydrodipicolinate synthase	spr0919	−2.1	−2.2	−2.9	−2.2
glyA	Serine hydroxymethyltransferase	spr0928	−2.0	−1.7	−1.4	−1.2
metY-truncation	O-Acetylhomoserine sulfhydrylase, truncation	spr1095	−3.5	−5.5	−2.9	−2.8
leuD-truncation	3-Isopropylmalate dehydratase small subunit, truncation	spr1133	2.0	1.2	1.5	1.2
gdhA	NADP-specific glutamate dehydrogenase	spr1181	−3.1	−3.5	−1.7	−2.0
metB	Cystathionine gamma-synthase	spr1377	−2.9	−3.2	−2.1	−1.8
metA	Homoserine O-succinyltransferase	spr1434	−2.1	−1.4	−1.1	−1.8
trpA	Tryptophan synthase alpha chain	spr1631	−2.0	−2.0	−1.3	−2.0
trpC	Indole-3-glycerol-phosphate synthase	spr1634	−2.2	−2.1	−1.3	−2.0
trpD	Anthranilate phosphoribosyltransferase	spr1635	−2.4	−2.4	−1.4	−2.3
trpG	Anthranilate synthase component II (glutamine amido-transferase)	spr1636	−5.1	−6.1	−6.0	−12.0
ilvD	Dihydroxyacid dehydratase	spr1935	−2.5	−2.2	−1.8	−1.8

^aMicroarray analyses were performed as described in Materials and Methods. Only genes whose transcript amounts showed a relative change of ≥2-fold in two independent experiments in response to at least one of the translation inhibitors are listed (see Materials and Methods). Negative values indicate a decrease in average relative transcript amount.

^bErm, erythromycin; Cm, chloramphenicol; Pur, puromycin; Tet, tetracycline, at concentrations shown in Table 1.