TABLE 3.
Genes with altered transcript amounts in a ΔpurR::aad9 mutant compared to its isogenic purR+ parent
| Functional group and R6 genome entry no. | Gene | Avg fold change in ΔpurR mutanta | Protein |
|---|---|---|---|
| Purine gene cluster | |||
| spr0045 | purC | 13.5 | Phosphoribosylaminoimidazole-succinocarboxamide synthetase |
| spr0046 | purL | 26.2 | Phosphoribosylformylglycinamide synthetase |
| spr0047 | purF | 12.5 | Amidophosphoribosyl transferase |
| spr0048 | purM | 10.6 | Phosphoribosylaminoimidazole synthetase |
| spr0049 | purN | 14.6 | 5′-Phosphoribosylglycinamide transformylase 1 |
| spr0050 | vanZ | 18.6 | Teicoplanin resistance protein |
| spr0051 | purH | 23.4 | Phosphoribosylaminoimidazolecarboxamide formyltransferase |
| spr0052 | purD | 8.7 | Phosphoribosylglycinamide synthetase |
| spr0053 | purE | 5.0 | Phosphoribosylaminoimidazole carboxylase, catalytic subunit |
| spr0054 | purK | 12.4 | Phosphoribosyl glucinamide formyltransferase |
| spr0055 | spr0055 | 4.7 | Hypothetical protein |
| spr0056 | purB | 3.9 | Adenylosuccinate lyase |
| Purine transport and salvage | |||
| spr0264 | spr0264 | 6.5 | Guanine/hypoxanthine transporter? |
| spr1128 | guaC | 5.5 | GMP reductase |
| spr1662 | xpt | 3.0 | Xanthine phosphoribosyltransferase |
| spr1663 | pbuX | 3.2 | Nucleobase:cation symporter for xanthine |
| Folate metabolism | |||
| spr0266 | sulA | 2.5 | Dihydropteroate synthase |
| spr0267 | sulB | 2.6 | Dihydrofolate synthetase |
| spr0268 | sulC | 2.5 | GTP cyclohydrolase |
| spr0269 | sulD | 2.1 | Aldolase-pyrophosphokinase |
| spr1109 | fhs | 2.2 | Formate-tetrahydrofolate ligase |
| Hypothetical and other | |||
| spr0105 | spr0105 | 2.6 | Hypothetical transporter-truncation |
| spr0265 | spr0265 | 6.5 | Conserved hypothetical protein |
| spr0639 | copY | 2.7 | COPAB ATPases metal-fist-type repressor |
| spr0641 | ctpA | 2.2 | P-type ATPase, probable copper transporter |
| spr1440 | spr1440 | 2.5 | Conserved hypothetical protein |
| spr1441 | oxlT | 3.6 | Major facilitator:oxalate:formate antiporter |
| spr1792 | lysA | −3.9 | Diaminopimelate decarboxylase |
| spr1793 | purR | −27.2 | Repressor of purine biosynthetic genes |
a
Microarray analyses were performed as described in Materials and Methods on bacteria grown in CDM to mid-exponential phase. Only genes whose transcript amounts showed a relative change of ≥2-fold in three independent experiments are listed. A negative value indicates a decrease in average relative transcript amount.