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Expression of a yqfS-lacZ translational fusion during growth and sporulation of B. subtilis. B. subtilis PERM317 was grown to sporulation in liquid DSM (▪). Samples were collected at different times and treated with lysozyme, and the extracts were assayed for either β-galactosidase (♦) or GDH (•) activity. The β-galactosidase activity inside of the forespore lysozyme-resistant fraction (▴) was assayed as described in Materials and Methods.
