FIG. 6.

Northern blot (A) and RT-PCR analysis (B) of yqfS transcription during vegetative growth and sporulation of B. subtilis sigGΔ1 (strain WN118). (A) B. subtilis WN118 was grown in liquid DSM. Total RNA was isolated (35) during the times indicated (in hours). Samples (20 μg) were separated on agarose-formaldehyde gels (lower panel) and transferred to nylon membranes. The membrane was hybridized with a ³²P-labeled 1,181-bp fragment encompassing the entire yqfS sequence as described in Materials and Methods. (B) RNA samples (1 μg) isolated at the times indicated (in hours) from a B. subtilis sigGΔ1 DSM culture were processed for RT-PCR analysis as described in Materials and Methods. For the wild type (WT), the RNA was isolated from B. subtilis 168 (Fig. 5); FW was obtained with the forward primer in the absence of RNA, and RV was obtained with the reverse primer in the absence of RNA. The arrowhead shows the size of the expected RT-PCR product.