TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype and/or phenotypea | Source (reference) |
|---|---|---|
| Strains | ||
| B. subtilis | ||
| 168 | trpC2 | Laboratory stock |
| WN118 | sigGΔ1 trpC2 | Wayne Nicholson |
| PERM317 | trpC2 yqfS-lacZ; Cmr | This study |
| PERM336 | sigGΔ1 trpC2 yqfS-lacZ | This study |
| YB3000 | metB5 trpC2 xin- 1 sigB amyE (deleted for spβ) pCCR202 (recA-lacZ at amyE); Cmr | R. E. Yasbin |
| E. coli | ||
| SURE | e14−(McrA−) Δ(mcrCB-hsdSMR-mrr)171 endA1 supE44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5 (Kanr) uvrC [F′ proAB laclq lacZΔM15 Tn10 (Tetr)] | Stratagene |
| PERM162 | E. coli SURE, pUC18; Ampr Tcr | This study |
| PERM253 | E. coli SURE, pPERM253; Ampr Tcr | This study |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac/F′ proAB lacIqZΔ M15 Tn10 (Tetr) | Stratagene |
| PERM267 | E. coli XL1-Blue, pPERM253; Ampr | This study |
| XL10-Gold Kan | Tetr Δ(mcrA) 183, Δ(mcrCB-hsdSMR-mrr); 173 endA1 sup E44 thi-1 recA1 gyrA96 relA1 lacHte [F′ proAB lacIqZΔM15 Tn10 (Tetr) Tn5 (Kanr) Amy] | Stratagene |
| PERM348 | E. coli XL-Gold, pPERM348, Ampr | This study |
| Plasmids | ||
| pJF751 | Integrational lacZ fusion vector; Cmr | W. Nicholson (17) |
| pUC18 | Multisite E. coli cloning vector | Laboratory stock (63) |
| pPERM253 | yqfS gene cloned in pUC18 | This study |
| pPERM267 | 514-pb EcoRI/NaeI fragment of yqfS from pPERM253 cloned in pJF751 | This study |
| pPERM348 | yqfS ORF cloned into the BamHI site of PQE-30 | This study |
a
Cmr, chloramphenicol resistant; Ampr, ampicillin resistant; Tcr, tetracycline resistant.