FIG. 8.

Biosafe Coomassie blue staining (A) and radioactivity (B) of native PAGE gel. The positions of GST-*PhoR, His10-PhoP_WT, and His10-PhoP_R113E are indicated on the left. GST-*PhoR and His10-PhoP_R113E or His10-PhoP_WT (1 μg each) were mixed with or without 10 μCi of [γ-³²P]ATP in phosphorylation buffer. After incubation at room temperature for 20 min, the mixture was loaded onto the native gel. Lanes 1, GST-*PhoR with His10-PhoP_R113E in the presence of [γ-³²P]ATP; lanes 2, GST-*PhoR with His10-PhoP_R113E; lanes 3, GST-*PhoR with His10-PhoP_WT in the presence of [γ-³²P]ATP; lanes 4, GST-*PhoR with His10-PhoP_WT.