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PCR amplification of pcpD and the three genes known to be required for degradation of PCP in the wild-type strain S. chlorophenolicum strain RA-2 (lanes 1, 3, 5, and 7) and the pcpD knockout (lanes 2, 4, 6, and 8). Lanes 1 and 2, pcpA; lanes 3 and 4, pcpB; lanes 5 and 6, pcpC; lanes 7 and 8, pcpD. Lane 9 contains size markers.
