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Sequestration of free E by His6-σA and His6-σB. Immobilized His6-σA was used as a sensor in SPR experiments to determine the concentration of free E after incubation of a constant amount of E with different amounts of His6-σA or His6-σB. The percentage of maximal sensorgram height was plotted against the log10 σ factor concentration. Symbols indicate binding of σA to 100 nM E (○) or 50 nM E (▵) and binding of σB to 100 nM E (•) and 50 nM E (▴).
