Sequence of the rrnB P2 promoter region from −112 to +21. The most frequent rrnB P2 transcription start site is designated +1. The −10 and −35 hexamers are indicated in bold, start sites are underlined, and the upstream (−112, −68, −53, and −37) or downstream (+7 and +21) endpoints used for construction of lacZ fusions are indicated by ⌈ or ⌉, respectively. The −15C insertion is indicated by the symbol ^, and substitutions are identified below the arrows.