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. 2003 Jan;185(1):28–34. doi: 10.1128/JB.185.1.28-34.2003

FIG. 6.

FIG. 6.

rrnB P2 promoter activity is inhibited by the presence of extra rRNA operons. Lysogens containing promoter-lacZ fusions were transformed with a multicopy plasmid containing either an intact rrnB operon (pNO1301) or an rrnB operon containing a large deletion in the 16S and 23S rRNA genes (pNO1302). Promoter activity is expressed as the ratio of the β-galactosidase activity from a promoter-lacZ fusion in a strain containing pNO1301 to that in a strain containing pNO1302 and then normalized to the value obtained from a control lacUV5 promoter as previously described (12). RLG4993, lacUV5 (−59 to +36); RLG3848, rrnB P1 (−61 to +50); RLG5014, rrnB P2(−112 to +7); RLG3987, rrnB P2(−112 to +7; C−5A,A−4T,C−3A); RLG3915, rrnB P2(−112 to +7, C+5G,C +7G). The average results and standard deviations of experiments with three independent transformants are shown.