TABLE 1.
Strains, plasmids, and promoter constructs used in this study
| Strain or plasmid | Genotype or description | Source |
|---|---|---|
| Strains | ||
| VH1000 | MG1655 pyrE lacl lacZ (RLG3499) | 9 |
| CF1693 | relA251::kan spoT207::cam (RLG857) | 10, 39 |
| RLG3848 | VH1000 λrrnB P1(−61 to +50)-lacZ | This work |
| RLG3851 | VH1000 λrrnB P2(−37 to +7)-lacZ | This work |
| RLG3863 | VH1000 λrrnB P2(−52 to +7)-lacZ | This work |
| RLG3866 | VH1000 λrrnB P2(−37 to +7)-lacZ relA spoT | This work |
| RLG3871 | VH1000 λrrnB P1P2(−152 of P1 to +7 of P2)-lacZ | This work |
| RLG3897 | VH1000 λrrnB P2(−112 to +7; C-5A, A-4T, C-3A)-lacZ | This work |
| RLG3898 | VH1000 λrrnB P2(−112 to +7; Cins−15)-lacZ | This work |
| RLG3914 | VH1000 λrrnB P2(−112 to +21)-lacZ | This work |
| RLG3915 | VH1000 λrrnB P2(−112 to +7; C+5G,C+7G)-lacZ | This work |
| RLG4296 | VH1000 λrrnB P2(−68 to +7; C+5G,C+7G)-lacZ | This work |
| RLG4757 | VH1000 λrrnB P1(−152 to +50)-lacZ | This work |
| RLG4993 | VH1000 λlacUV5 (−59 to +36)-lacZ | This work |
| RLG5014 | VH1000 λrrnB P2(−112 to +7)-lacZ | This work |
| RLG6982 | VH1000 λrrnB P2(−112 to +7)-lacZ relA | This work |
| Plasmids | ||
| pRLG770 | General transcription vector | 29 |
| pRLG3858 | pRLG770 rrnB P1 (−152 of P1 to +7 of P2) | This work |
| pRLG3859 | pRLG770 rrnB P2 (−52 to +7) | This work |
| pRLG3865 | pRLG770 rrnB P2 (−37 to +7) | This work |
| pRLG3890 | pRLG770 rrnB P2 (−112 to +7; C-5A, A-4T,C-3A) | This work |
| pRLG3893 | pRLG770 rrnB P2 (−112 to +7; Cins−15) | This work |
| pRLG3895 | pRLG770 rrnB P2 (−112 to +21) | This work |
| pRLG3896 | pRLG770 rrnB P2 (−112 to +7; C+5G,C+7G) | This work |
| pRLG4871 | pRLG770 rrnB P2 (−112 to +7) | This work |
| pNO1301 | pBR322 expressing an intact rrnB operon | 14 |
| pNO1302 | pNO1301 with 2.4-kb deletion within 16S and 23S genes | 14 |