TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant propertiesa | Source or reference |
|---|---|---|
| S. enterica serovar Typhi strains | ||
| Ty2 | Wild type | ISPb |
| M985 | Ty2 Δwzz | This study |
| M1224 | Ty2 Δwzy | This study |
| MSS1 | Ty2 ΔwbaP::cat Cmr | 49 |
| M8 | Ty2 ΔrfaH::cat Cmr | 47 |
| M115 | Ty2 ΔwaaK | This study |
| M102 | Ty2 ΔwaaJ | This study |
| M1015 | Ty2 ΔwaaI | This study |
| M109 | Ty2 ΔwaaB | This study |
| M108 | Ty2 ΔwaaB::aph Kanr | This study |
| M113 | Ty2 ΔwaaG | This study |
| Plasmids | ||
| pKD46 | bla PBADgam bet exo pSC101 oriTS | 8 |
| pKD3 | bla FRT cat FRT PS1 PS2 oriR6K | 8 |
| pKD4 | bla FRT aph FRT PS1 PS2 oriR6K | 8 |
| pCP20 | bla cat cI857 λPRflp pSC101 oriTS | 5 |
| pGEM-T Easy | TA cloning vector | Promega |
| pJC102 | waaJ cloned into pGEM-T Easy | This study |
| pJC1015 | waaI cloned into pGEM-T Easy | This study |
| pJC109 | waaB cloned into pGEM-T Easy | This study |
| pJC113 | waaG cloned into pGEM-T Easy | This study |
a
Cmr, chloramphenicol resistant; Kanr, kanamycin resistant.
b
ISP, Institute of Public Health, Santiago, Chile.