FIG. 5.

Intestinal localization of terminal signal. The mouse shown was infected with attenuated strain 2C(Δhly) and imaged until symptoms were gone but the gallbladder signal was retained (day 3). After fasting for 6 h, the animal was then injected intraperitoneally with CCK8, and serial images were taken as described in the legend of Fig. 3. When the signal was stationary for several consecutive images, the animal was sacrificed and the intestine was removed for ex vivo BLI. Images of the excised organ were then taken at a higher resolution (binning, 2 by 2) (B) and a higher signal-to-noise ratio (binning, 10 by 10) (C). Multiple reference images were used for the in vivo series. Quantification of the signals using regions of interest identical to those of Fig. 2 is shown in the bottom panel.