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Changes in cytokine profiles. The BALB/c mice were immunized with pcDNA3/fliC, pcDNA3/CpG-fliC, or pcDNA3. The immunized mice, which were seropositive for flagellin, were infected with 105 CFU of B. pseudomallei by intravenous injection in the tail. Subsequent to the indicated time delay (in days), extracted spleen cells were used to examine the intracellular level of cytokine-specific mRNA by RT-PCR.
