TABLE 1.
Salmonella operons with high expression during enteritis
| First locusa | Gene | Operonb | Function (classificationc) | Expression leveld
|
Previous evidence for expression/attenuatione (reference) | |
|---|---|---|---|---|---|---|
| Enteritis | Typhoid fever | |||||
| STM3668 | yiaK | yiaK-O-lyxK-sgbHUE | Uptake and utilization of lyxose ascorbate (P) | 470 | 30 | NA |
| STM4501 | STM4501-4500 | Putative cytoplasmic protein, putative SAM-dependent methyltransferase (U) | 380 | 40 | NA | |
| STM4315 | rtsA | rtsAB | Regulator of SPI-1 expression (V) | 290 | b.t. | NA |
| STM0974 | focA | focA-pflB | Formate transporter, pyruvate formiate lyase (P) | 300, 210 | b.t. | exp (78) |
| STM2283 | glpT | glpTQ | Glycerol transporter (P) | 240 | 30 | exp (15) |
| STM2344 | STM2344-2340 | PTS system, utilization of unknown carbohydrates (P) | 210 | 40 | NA | |
| STM0744 | ybgC | ybgC-tolQRAB-pal-ybgF | Maintenance of cell envelope integrity (P) | 210 | 35 | att* (tolB) (8) |
| STM3881 | rbsD | rbsDACBK | Ribose ABC transporter (P) | 230, 170 | 40, 35, 30, 25 | exp (15) |
| STM0608 | ahpC | ahpCF | Alkylhydroperoxide reductase (P) | 180 | b.t. | NA |
| STM2876 | hilA | hilA-iagB | Regulator of SPI-1 expression, cell invasion protein (V) | 270, 120 | b.t. | att (3, 69) |
| STM1328 | Putative outer membrane protein (U) | 170 | b.t. | NA | ||
| STM2328 | nuoA | nuoA-N | NADH dehydrogenase I (P) | 140 | 50 | NA |
| STM2875 | hilD | Regulator of SPI-1 expression (V) | 120, 100, 90, 85, 85, 80, 80 | 65, 45 | NA | |
| STM2330 | lrhA | Regulation of flagella, motility, chemotaxis genes (P) | 160, 130, 80, 70 | 15, b.t. | NA | |
| STM2886 | sicA | sicA-sipBCDA-iacP | SPI-1 effector proteins and chaperons (V) | 120, 90 | b.t. | att (3, 69) |
| STM1155 | htrB | Lipid A biosynthesis (P) | 105 | b.t. | NA | |
| STM0260 | dniR | Regulator of nitrite reductase (P) | 90 | 40 | NA | |
| STM0191 | fhuA | fhuACDB | Ferrichrome uptake system (P) | 130, 45 | b.t. | exp (38) |
| STM2867 | hilC | Regulator of SPI-1 expression (V) | 40 | b.t. | NA | |
| STM4423 | Putative AraC-type DNA-binding domain-containing protein (U) | 35 | b.t. | NA | ||
| STM2079 | wzzB | LPS synthesis (P) | 35, 20 | 15 | NA | |
First locus downstream of the identified promoter. Loci with the prefix STM were annotated by McClelland et al. (53).
Predicted operons according to published data and operon prediction at TIGR.
Classification into functional categories: P, physiological; V, virulence; U, unknown function.
GFP_OVA levels in ceca of infected streptomycin-pretreated mice or in spleens of intravenously infected mice in 1,000 copies per Salmonella cell (each value represents one infected mouse); b.t., below detection threshold of 10,000 GFP copies per cell in spleens of infected mice.
exp, expression in gut; att, inactivation of the operon leads to loss of enteritis phenotype in mice or cattle; att* (tolB), inactivation results in tolB-dependent attenuation in typhoid fever model; NA, no information available.