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. 2006 Mar;74(3):1643–1648. doi: 10.1128/IAI.74.3.1643-1648.2006

FIG. 2.

FIG. 2.

Exposure of T. cruzi gp83 ligand to HCASM cells up-regulates the expression of laminin γ-1. (A) Exposure of gp83 to HCASM cells up-regulates the expression of laminin γ-1 as determined by cDNA microarray analysis. RNA from HCASM cells exposed or not exposed to gp83 was reverse transcribed to labeled cDNA and hybridized onto cDNA microarray slides. Duplicate hybridizations were normalized against their respective controls and analyzed with GeneSpring Software. Bars represent the mean ± 1 standard deviation of ECM gene expression of duplicate experiments with similar results. *, significant difference between laminin γ-1 gene expression and the expression of the other ECM genes (P < 0.01). (B) Kinetics of laminin γ-1 transcript upon exposure of gp83 to HCASM cells. RNA from cells exposed or not exposed to gp83 was reversed transcribed to cDNA, and the laminin γ-1 transcript levels were evaluated by real-time-PCR. Data are the mean ± 1 standard deviation of normalized levels of laminin γ-1 transcript against human 18S RNA in one representative experiment of three independent experiments performed in triplicate with similar results. Each point is the mean of results for triplicate samples in one representative experiment (±1 standard deviation). The P value was <0.05 for points at 120 and 180 min with respect to points at 0 and 60 min.