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Laminin γ-1 RNAi constructs used for generating shRNA in HeLa cells. (A) Schematic representation of the predicted shRNA structure. The orientation of the oligonucleotide sequences cloned behind a strong U6 promoter is shown. (B) Design of oligonucleotide constructs from laminin γ-1 cDNA to generate shRNA. The 5′ and 3′ termini underlined show the modification for cloning into the IMG-800 vector. Numbers on oligonucleotides show the locations on the laminin γ-1 cDNA.
