TABLE 1.
Relative in vivo fitness of a B. pertussis heme utilization mutant
| Time postinfection (days) | Mean CIa
|
Mean CI differencec (CIno hemin − CIwith hemin) | |
|---|---|---|---|
| No hemin coadministration | With heminb coadministration | ||
| 3 | 0.90 ± 0.15 (0.1990) | 0.80 ± 0.12 (0.0223) | 0.102 (0.0003) |
| 7 | 0.91 ± 0.14 (0.2396) | 0.66 ± 0.11 (0.0021) | 0.252 (<0.0001) |
| 14 | 0.36 ± 0.05 (<0.0001) | 0.27 ± 0.03 (<0.0001) | 0.082 (0.0001) |
| 21 | 0.24 ± 0.03 (<0.0001) | 0.25 ± 0.03 (<0.0001) | −0.002 (0.3739) |
| 28 | 0.11 ± 0.01 (<0.0001) | 0.11 ± 0.01 (<0.0001) | 0.000 (—d) |
Mean CI value ± standard deviation, n = 5 mice (P value). P value is the probability that the mean CI value at each time point postinfection is the same as the reference value of 1.00 (the predicted mean CI if there were no difference in multiplication and survival between the two strains used in mixed infections). The test used was the one-sample t test with a hypothesized mean of 1.00. Probabilities (P) of ≤0.05 were considered significant.
Bovine hemin chloride (2 μM) coadministered with B. pertussis.
Mean CI difference between “no hemin” and “with hemin” infection groups (P value). P value is the probability that the mean CI for infections with 2 μM hemin coadministration is the same as the mean CI for infections without hemin coadministration at each time point postinfection. The test used was the paired t test with a hypothesized difference of 0.00. Probabilities (P) of ≤0.05 were considered significant.
No difference in mean CI.