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. 2006 Mar;74(3):1745–1750. doi: 10.1128/IAI.74.3.1745-1750.2006

FIG. 1.

FIG. 1.

Expression and purification of conserved and variable regions of LigA. The conserved and variable regions of LigA were cloned into pGEX4T-2 and expressed as GST fusion proteins as described in Materials and Methods. GST fusion protein was purified by affinity chromatography and subjected to SDS-PAGE followed by Coomassie blue staining. (A) Expression of the conserved region of LigA. (B) Expression of the variable region of LigA. Lane 1, E. coli with vector and pGEX4T-2 only (control); lane 2, uninduced E. coli with recombinant construct; lane 3, IPTG-induced E. coli with recombinant construct; lane 4, affinity chromatography-purified GST fusion proteins (∼1.5 μg). M represents the molecular size marker in kilodaltons (Bio-Rad).