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Gene-specific PCR products showing levels of expression of TLR2, TLR4, and TLR15 mRNAs in a panel of tissues from healthy chickens. Total RNA was extracted from 5 to 7 chickens, and cDNAs were made and amplified as described in the text. 18S RNA was used as an internal positive control for normalization. Negative controls (NC) contain no cDNA template.
