FIG. 3.

Determination of K_m and k_cat values for 4-coumaroyl-CoA formation by ScCCL. The initial reaction velocities (v) of 4-coumaroyl-CoA formation by ScCCL were determined from five independent experiments. The kinetic constants K_m for 4-coumarate and k_cat were estimated by linear regression of 1/v against 1/[S] (Lineweaver-Burk plot) at fixed concentrations of ATP (5 mM) and CoA (0.3 mM). The plot gave a line with the x intercept equal to −1/K_m and with the y intercept equal to 1/k_cat [ScCCL]₀.