FIG. 5.

Characterization of PanNK⁺ T cells in the livers of acutely T. cruzi-infected C57BL/6, BALB/c, A/J, and C3H/HePAS mice. Seven and fourteen days after infection, intrahepatic lymphocytes were isolated from perfused livers and analyzed by fluorescence-activated cell sorting. Noninfected animals were used as controls. In panel A, total numbers per liver of CD3⁺, CD4⁺, CD8⁺, and CD4⁻ CD8⁻ cells expressing PanNK in the four mouse strains are given. Results represent the mean ± SD (n = 3) of a representative experiment (out of three experiments). In panel B, percentages of large cells among PanNK⁺ CD3⁺ cells in the four mouse strains are given. Results represent the mean ± SD (n = 3) of a representative experiment (out of three experiments). In panel C, dot plots of a representative C57BL/6 mouse show the coexpression of PanNK and NK1.1 among gated CD3⁺ cells. Mean percentages (n = 3) of cells in each region from the same experiment (out of three experiments) are shown.