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Impaired BLC gene induction in NIK−/− cells. BMDM were stimulated for 24 h with LPS (0.02 to 0.4 ng/ml) and 20 μg/ml MDP. Gene induction (mRNA) was determined by real-time PCR. The relative expression of genes was normalized to the level of 18s RNA. Data are presented as means ± standard deviations of triplicate wells. **, P < 0.01 for cytokine concentrations from knockout mice compared with data from wild-type mice. Results are representative of two independent experiments.
