FIG. 2.

Nramp1 relative expression levels in monocytes from AA, AB, and BB monocytes, noninfected and infected (4 h) with Brucella abortus 2308. The CD64 gene was used as an internal standard. Relative expression levels were calculated by the comparative cyclic threshold method (16). Each genotypic class includes three animals, each animal tested in triplicate. Nramp1 expression level in BB-induced monocytes is significantly different (P < 0.01) from the level in BB noninduced monocytes and from the levels in AA and AB monocytes, induced and noninduced. Nramp1 expression level in BB noninduced monocytes is significantly different (P < 0.05) from the levels in AA and AB monocytes, induced and noninduced.