TABLE 1.
Effect of immunization on P. carinii burden
| Immunogen | P. carinii burdena | No. of animals immunized | No. of animals with detectable P. carinii |
|---|---|---|---|
| A12:THIO | 4.83 × 104 ± 9.81 × 104b | 14 | 4c |
| THIO | 3.52 × 105 ± 3.42 × 105 | 10 | 8 |
a
The data are means ± standard deviations and are the P. carinii kex1 copy numbers obtained in triplicate analyses of boiled lung homogenates by real-time PCR. Mice with no detectable P. carinii as determined by real-time PCR were assigned a value of 9.99 × 103 copies, which was just below the limit of detection (1 × 104 copies).
b
The value is significantly different from the value for THIO-immunized mice as determined by Student's t test (P ≤ 0.005).
c
The number of mice with detectable P. carinii is significantly different from the number of THIO-immunized mice with detectable P. carinii (χ2 = 6.17; P ≤ 0.025).