FIG. 3.

Quantitation of TNF-α mRNA in spleens (A), renal lymph nodes (LN) (B), and popliteal lymph nodes (C). Gerbils were necropsied at 3 h, 24 h, 3 days, 7 days, and 28 days after i.d. inoculation with Brugia pahangi L3. mRNA levels were measured by RT-PCR, and values are expressed as the mean severalfold changes compared with values from control animals. Superscript letters indicate statistical significance (P < 0.05). Values with the same letter are not statistically different. No statistically significant differences were found in the spleen (P = 0.065) or popliteal lymph node (P = 0.2).