FIG. 2.

Visual evaluation of hard tissue changes on defleshed bone specimens. Defleshed bone specimens were stained with methylene blue to indicate the changes in bone level in the areas where periodontitis was induced. Similar to soft tissue changes, the red arrows depict significant bone loss on both aspects (buccal [B] and lingual [L]) of the teeth in groups B and C. Conversely, topical application of cimetidine before P. gingivalis application prevented bone destruction in a significant way with no apparent dose-dependent effect (bottom) (groups D, E, and F).