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Phagocytosis-mediated NOS 2 expression. RAW264.7 cells pretreated for 10 min with cytochalasin D (10 μg/ml) and infected with B. anthracis ΔgerH spores for 18 h were lysed, protein run on polyacrylamide gel, and subjected to immunoblot analysis for NOS 2 expression. Sample lysates were probed for α-tubulin as a protein loading control. Data are representative of three independent experiments.
