FIG. 5.

Differential NOS 2 induction in sonicated and nonsonicated B. anthracis. The removal of the B. anthracis exosporium by the process of sonication allowed examination of the differences in spore-host interactions mediated by the exosporium. (A) RAW264.7 cells infected with sonicated and nonsonicated B. anthracis ΔgerH spores (MOI, 1:1) at 37°C for 18 h were lysed, protein run on polyacrylamide gel, and subjected to immunoblot analysis for NOS 2 expression. Sample lysates were probed for α-tubulin as a protein loading control. (B) Quantitative analyses through immunoblot densitometry were performed utilizing the NIH ImageJ program. Data are representative of three independent experiments.