TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| S. gordonii | ||
| DL1 Challis | Wild type | 50 |
| 1.1L | luxS::ermAM (LuxS− Emr) | This study |
| 1.1LC | luxS::ermAM::pVA981luxS (LuxS+ Tcr Emr) | This study |
| P. gingivalis | ||
| ATCC 33277 | Wild type | ATCC type strain |
| PLM1 | luxS::pLR409 (LuxS− Emr) | 9 |
| E. coli | ||
| Top10 | F−mcrA (mrr-hsdRMS-mcrBC) φ80lacZΔM15 lac74 recA1 deoR araD139 Δ(ara leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| DH5α | F−deoR endA1 gyrA96 hsdR17 (rK− mK−) recA1 relA1 supE44 thi-1 Δ(lacZYA-argFV169) φ80lacZΔM15 | 26 |
| V. harveyi BB170 | luxN::Tn5lac (AI-2 reporter strain) | 1 |
| Plasmids | ||
| pVA736 | 7.6 kb; Emr, pVA380-Iori | 44 |
| pVA981 | 7.1 kb; Tcr, ColE1ori | 41 |
| pVALuxS | S. gordonii luxS in pVA981 | This study |
| pCRLux2 | S. gordonii luxS in pCR4-TOPO | This study |
| pCRLux2Em | S. gordonii luxS::ermAM in pCR4-TOPO | This study |