TABLE 3.
luxS gene inactivation does not affect the expression of the coaggregation adhesins SspA, SspB, or CshA in S. gordonii
| Antiserum (dilution) | Mean ELISA reactivitya ± SDb with:
|
||
|---|---|---|---|
| DL1 (LuxS+) | 1.1L (LuxS−) | 1.1LC (LuxS+) | |
| P1c (1:500) | 0.165 ± 0.020 | 0.166 ± 0.018 | 0.182 ± 0.008 |
| CshA (1:1,000) | 0.278 ± 0.008 | 0.263 ± 0.007 | 0.276 ± 0.017 |
a
Input 2 × 107 cells per well. Results (A492) were corrected for background by subtracting values obtained with preimmune serum diluted appropriately.
b
Standard deviations of the mean (n = 4).
c
P1 antiserum reacts with both SspA and SspB.