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. 2003 Jan;185(1):148–154. doi: 10.1128/JB.185.1.148-154.2003

FIG. 1.

FIG. 1.

(A) Diagram showing two possible P1-mediated crossover events between the donor (ompC234 [OmpC1Cys] zei::Tn10 or (ompC402 [OmpC2Cys] zei::Tn10) and the recipient (ompC′::lacZ+ degP::Kmr/pdegPS210A) DNA. The cotransductional linkage between the zei::Tn10 (the selectable marker) and ompC is 50%. A crossover between 1 and 2 will yield tetracycline-resistant (Tcr) Lac+ (ompC′::lacZ+) transductants, whereas a crossover between 1 and 3 will yield Tcr Lac− (ompC) transductants. (B) P1 transduction results drawn from diagram presented in panel A.