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In vivo degradation of a LamB amber fragment in various genetic backgrounds. Freshly grown cells were subjected to pulse-chase analysis as described in Materials and Methods. LamB and MalE (as a gel loading control) were immunoprecipitated from [35S]methionine-labeled protein extracts obtained from DegP+ (□), DegP− (▪), DegP+/DegPS210A (○), and DegP−/DegPS210A (•). The highest LamB level was taken as 100, and the other LamB values were adjusted accordingly.
