FIG. 5.

Sucrose density gradient analyses of whole-cell envelopes. Envelopes obtained from DegP⁺ OmpC_1Cys, DegP_S210A OmpC_1Cys, DegP⁺ OmpC_2Cys, and DegP_S210A OmpC_2Cys strains (panels 1 to 4, respectively) were fractionated into inner and outer membranes by sucrose density gradients. After centrifugation, fractions were collected from top and analyzed by SDS-PAGE. Protein bands were visualized by Coomassie blue staining. Fractions corresponding to inner membrane (IM), intermediate density material (M), and outer membrane (OM) are shown on top. Positions of OmpC, OmpA, DegP_S210A, and a protein band (○) that primarily localizes with the intermediate-density material are indicated.