FIG. 7.
Direct interaction between DegPS210A-6His and OmpC2Cys. Freshly grown cells expressing DegPS210A-6His and OmpC2Cys were lysed as described in Materials and Methods. Membrane-free cell lysates were subjected to Ni2+-nitrilotriacetic acid column chromatography to purify DegPS210A-6His. Four identical samples (lanes 1 to 4) of the eluted fraction containing DegPS210A-6His were subjected to SDS-PAGE and Western blot analyses with the indicated antibodies. An aliquot of outer membrane containing OmpC and OmpA proteins was analyzed in lane 5.