TABLE 1.
Strains used in this study
| Strain | Relevant characteristicsa | Source or reference |
|---|---|---|
| MC4100 | F−araD139 Δ(argF-lac)U139 rpsL150 flbB5301 ptsF25 deoC1 thi-1 rbsR relA | 4 |
| PLB3261 | MC4100 ΔlamB106 φompC′::lacZ+ | S. Benson |
| RAM412 | PLB3261 ompC234 (OmpC1Cys) zei::Tn10 (50% linkage to ompC by P1 transduction) | 12 |
| RAM415 | PLB3261 ompC402 (OmpC2Cys) zei::Tn10 | 12 |
| RAM1015 | MC4100 lamB677 (LamB amber) | Lab strain |
| RAM1025 | RAM1015 degP::Kmr | Lab strain |
| RAM1120 | RAM1025 pCS10 (DegPS210A) | This study |
| RAM1121 | RAM1025 pACYC184 | This study |
| RAM1122 | RAM1015 pCS10 | This study |
| RAM1123 | RAM1015 pACYC184 | This study |
| RAM1124 | PLB3261 degP::Kmr pCS10 | This study |
| RAM1125 | RAM412 degP::Kmr pCS10 | This study |
| RAM1126 | RAM415 degP::Kmr pCS10 | This study |
| RAM1127 | RAM415 recA::Kmr | This study |
| RAM1128 | RAM415 recA::Kmr pCS21 (DegPS210A-6His) | This study |
a
Kmr, kanamycin resistance.