Table 2.
Group | Surgery | Steroid hormone capsules | Steroid hormone injection (4-day cycle) | Treatment abbreviation |
---|---|---|---|---|
Males | ||||
1 | Sham-orchidectomy | Blank | Vehicle | Sham |
2 | Orchidectomy | Blank | Vehicle | GDX + 0 |
3 | Orchidectomy | Ta | Vehicle | GDX + T |
4 | Orchidectomy | E2 | Vehicle | GDX + E2 |
5 | Orchidectomy | DHT | Vehicle | GDX + DHT |
6 | Orchidectomy | E2 + DHT | Vehicle | GDX + E2/DHT |
Intact femalesb | ||||
7 | Sham-ovariectomy | Blank | Vehicle | Sham-diestrus-1 (D) |
8 | Sham-ovariectomy | Blank | Vehicle | Sham-proestrus (P) |
9 | Sham-ovariectomy | Blank | Vehicle | Sham-estrus (E) |
GDX females | ||||
10 | Ovariectomy | Blank | Vehicle | GDX + 0 |
11 | Ovariectomy | E2c | Vehicle | GDX + E2 |
12 | Ovariectomy | E2c | P4 | GDX + E2/P4 |
13 | Ovariectomy | Blank | P4 | GDX + P4 |
14 | Ovariectomy | T | Vehicle | GDX + T |
In GDX males, two doses of T were tested: ‘low’, one 2-mm capsule/100 g body weight; ‘high’, one 10-mm capsule/100 g body weight.
No sham females were tested during diestrus-2 because, in terms of gonadal steroid levels, reproductive behavior and reproductive physiology, there are few differences between diestrus-1 and -2 (Freeman, 1988).
In GDX females, two doses of E2 were tested: ‘low’, one 1-mm capsule/rat; ‘high’, one 5-mm capsule/rat.