Table 4.
Estimated percent time (mean ± 1 SEM) that female rats were in each phase of the estrous cycle, and the number of rats that were in each stage on the day of nociceptive testing (in parentheses)a
| % Time in each phase of estrous cycleb |
|||||
|---|---|---|---|---|---|
| Treatment group | Proestrus | Proestrus/estrus | Estrus | Diestrus-1 | Diestrus-2 |
| Sham | 14.0 ± 2.4 (7) | 7.4 ± 1.7 (0) | 17.0 ± 2.7 (10) | 41.9 ± 4.0 (9) | 20.6 ± 3.2 (0) |
| GDX + 0 | 0 ± 0 (0) | 0 ± 0 (0) | 0 ± 0 (0) | 80.0 ± 12.3 (10) | 20.0 ± 12.3 (1) |
| GDX + E2 (lowc) | 0 ± 0 (0) | 22.5 ± 10.1 (2) | 77.5 ± 10.0 (7) | 0 ± 0 (0) | 0 ± 0 (0) |
| GDX + E2 (highc) | 2.5 ± 2.7 (0) | 75.5 ± 12.9 (2) | 11.5 ± 6.0 (9) | 0 ± 0 (0) | 0 ± 0 (0) |
| GDX + E2 (low)/P4 | 18.3 ± 7.7 (2) | 10.0 ± 2.8 (2) | 50.0 ± 10.6 (4) | 21.7 ± 11.5 (0) | 0 ± 0 (0) |
| GDX + E2 (high)/P4 | 15.4 ± 4.5 (1) | 10.1 ± 2.4 (2) | 60.9 ± 10.2 (7) | 6.4 ± 2.7 (0) | 0 ± 0 (0) |
| GDX + P4 | 0 ± 0 (0) | 0 ± 0 (0) | 0 ± 0 (0) | 77.1 ± 7.7 (8) | 22.8 ± 7.7 (3) |
| GDX + T | 0 ± 0 (0) | 0 ± 0 (0) | 0 ± 0 (0) | 72.0 ± 6.1 (7) | 28.0 ± 6.1 (3) |
a
For treatment group abbreviations see Table 2.
b
Based on daily vaginal cytology sampled on 14 consecutive days, beginning on day 15 post-surgery (except sham females, in which the average sample was based on 10 days, because some females were tested before day 28).
c
Low, 1-mm capsule/rat; high, 5-mm capsule/rat.