Table 2.
Bovine markers developed for RH mapping
| STS-EST | SEQUENCE UP | SEQUENCE DN | SIZE OF PCR FRAGMENT | % RETENTION ON RH5000 | POSITION (cR) | HUMAN LOCATION (Mb) |
| KHDRBS2 | ATGGGGTTGCAAACAAGAAG | TTTGACAAATTGTGGTTTCCT | 300 | 36 | 0 | 62.75 |
| PRIM2A | AAAGCAGCATTCTGGACTGG | CCAGAAAAGGTCTGGGTCAA | 104 | 40 | 82 | 57.29 |
| BAG2 | AATCTGACTGCGAACCGTCT | GTTTCCCAGGTCATCCAGAA | 150 | 36 | 94 | 57.15 |
| DST | CCACCTGGACCTGAGACCTA | ACAGGCGCTACTGTCCACTC | 101 | 39 | 106 | 56.58 |
| COL21A1 | CAGTTCTTCGGAGCCTTGAG | GACTGCAACTGACTGGCTGA | 133 | 34 | 125 | 56.12 |
| TINAG | ACCGCATAGCAATTCAGTCC | ACCACCAAGCCCTATCAACA | 123 | 31 | 184 | 54.28 |
| LANO | CGGTCTCCAGCTTGATTGTT | TCCAAGAGAAGGTGGGTTTG | 185 | 37 | 202 | 53.77 |
| RAB2L | TCAATCTGGTCCCCTCTCAG | CCAGGACAGAGCTGACCACT | 141 | 33 | 230 | 33.37 |
| B3GALT4 | GGAGGCAAGCACCAGGTAT | CTGGCCACCTTCAGGATAAG | 125 | 37 | 33.35 | |
| RING1 | ATCCAAAGTGACCCCACAAG | GGCAATAGAAAAGGCAGCAG | 228 | 37 | 33.29 | |
| HKE4 | GACTATGTCCGTGGTGCAAA | CTGTACGAAGACCTGCACGA | 181 | 37 | 33.28 | |
| RXRB | CTGAAAAAGGTGGTGGTGGT | CATAAGAGCCGCAGAGAACC | 193 | 37 | 33.27 | |
| VPS52 | ACCCAGCTGATCCAGCTCTA | ATCAGAAGTTGGGCTTGTGC | 138 | 29 | 33.33 | |
| ELOVL5 | TCATGCTTTGATTTTGCACAT | TGTAAACACGAAGCCGTGACT | 104 | 28 | 53.28 | |
| PTP4A1 | GTGATTCCCAGCCTCTCTTG | CCACTTGTTCCCGGACAGTA | 199 | 50 | 64.35 | |
| PHF3 | CCATTTCGAAGAGGATCAGC | AACCCAGGTGGGCTAGACTT | 188 | 37 | 64.45 |
Markers in bold belong to the framework RH map whereas markers in italic are positionned with the best likelihood. Primer sequences, retention fraction, RH map location on bovine map and human location are indicated.