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Overexpression of DYRK1A increases the phosphorylation of endogenous sites in COS-7 cells. GFP-SF3b1-NT was expressed in COS-7 cells either alone (A) or coexpressed with GFP-DYRK1A (B) and metabolically labelled with 32P. SF3b1 fusion proteins were subjected to phosphopeptide mapping as above. Intensities of peptide maps A and B were adjusted to spot X/1.
