Skip to main content
. Author manuscript; available in PMC: 2006 Mar 30.
Published in final edited form as: Mol Cancer Res. 2005 Nov;3(11):617–626. doi: 10.1158/1541-7786.MCR-05-0146

Figure 1.

Figure 1

Hairpin genomic sequencing methodology. (A) Hairpin GS of the NBL2 repeat with the covalently linked upper and lower strands (not to scale) diagrammed as a hairpin to illustrate their complementarity before bisulfite deamination of all unmethylated C residues. mC, 5-methylcytosine. The recognition site for BsmAI is in italics and its cleavage specificity is shown on the right. (B) Examples of discrimination between different methylated configurations of CpG dyads and C-to-T changes in a genomic sequence. Part of one strand of each molecular clone is depicted in the hairpin configuration to align CpG positions that were part of a genomic dyad.