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. 2006 Apr 4;4(4):e115. doi: 10.1371/journal.pbio.0040115

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Copyright: © 2006 Mikels and Nusse.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Wnt5a protein treatment does not affect cytosolic β-catenin protein levels. The 293 cells were treated for 3 h with the indicated concentrations of Wnt proteins and then hypotonically lysed for Western blot analysis of cytosolic fractions.

(B) Wnt5a protein treatment does not alter β-catenin cellular localization. Cells grown on coverslips were treated with the indicated concentrations of Wnt proteins for 6 h and then fixed and stained as described in the text. Membrane localized β-catenin is indicated by arrowheads; membrane and cytosolic staining is indicated by arrows.