Figure 5. A. Reverse transcription–polymerase chain reaction of mRNA for TNF in splenic tissue collected 2 hours after sham conditioning, conditioning SLH, and SLH preceded by macrophage inhibition with CNI-1493. BMG indicates the housekeeper gene β-2 microglobulin. B. Graphic representation of above reverse transcription–polymerase chain reaction. Ratio of TNF and BMG cDNA is used to control for loading and variations in amplification. ^*, p < 0.05 SLH vs. sham and SLH + CNI-1493.