Figure 5. Hoechst dye staining of normal rat skin and wounded tissue. In normal rat skin (A), Hoechst dye stain was present in the epithelial layer (A1), sebaceous glands (A2), and hair follicles (A3). At day 1 (B), the wounded tissue was well perfused and the newly generating epithelial layer (B4) and provisional fibrin matrix (B5) exhibited the nuclear stain. Orientation of the figures is shown as outer layer (OL) and wounded tissue (WT).