Figure 2. Neutrophils adherent to uncoated or plasma-coated (A) expanded polytetrafluoroethylene (ePTFE) and (B) Dacron exhibit a time-dependent increase in cell death. Neutrophils were plated onto polystyrene plates uncoated (control) or precoated with fetal bovine serum (control + FBS) or plasma (control + plasma). Parallel wells were prelined with ePTFE or Dacron. The ePTFE and Dacron surfaces were then untreated or plasma-coated. At 1, 3, and 6 hours, neutrophils were stained with SYTO 9 and propidium iodide (PI). Fluorescent cells in random microscopic fields were counted as described in the legend to Figure 1, and the percentage of PI-positive cells relative to the total neutrophil population was determined. Data are expressed as mean ± SEM from four independent experiments. *Significant difference (P < .001) between the percentage of PI-positive neutrophils adherent to untreated versus plasma-coated Dacron surfaces.