Figure 3. (A) Neutrophils adherent to expanded polytetrafluoroethylene (ePTFE) and Dacron exhibit a time-dependent increase in cell death as determined by lactate dehydrogenase (LDH) release. Neutrophils were plated onto plates precoated with fetal bovine serum (control), (B) fibrinogen (FBGN) or immunoglobin (IgG), or prelined with ePTFE and Dacron. As a positive control, neutrophils plated onto a subset of control wells were treated with phorbol ester (PMA) at the time of plating. At 1, 3, and 6 hours, supernatants were collected and analyzed for LDH release. The amount of LDH released by neutrophils plated onto control wells for 1 hour served as a point of reference for each experiment. Data are expressed as a ratio relative to the point of reference. Mean values ± SEM from four independent experiments are shown.