Figure 6. Lack of DNA fragmentation in DNA samples isolated from neutrophils adherent to Dacron and expanded polytetrafluoroethylene (ePTFE). DNA was isolated from untreated neutrophils (A, UN, lane 2), neutrophils adherent to ePTFE for 1 hour (A, ePTFE, lane 3), neutrophils cultured for 24 hours at 37°C to render them apoptotic (A and B, apoptotic, lanes 4 and 2), or neutrophils adherent to Dacron (B, lane 4) and ePTFE (B, lane 3) for 6 hours. After isolation, equal DNA amounts were resolved on agarose gels and visualized by ethidium bromide staining. A 123-bp DNA ladder was resolved in A, lane 1. A low-molecular-weight DNA ladder was resolved in B, lane 1. Fragmented DNA was seen only in the apoptotic groups.